EZH2 immunoexpression in pleomorphic adenoma and adenoid cystic carcinoma and clinicopathological features

Abstract The aim of this study was to evaluate the expression of the EZH2 protein and describe the clinical and microscopic characteristics of adenoid cystic carcinoma (ACC) and pleomorphic adenoma (PA). The study included 16 ACC cases and 12 PA. All ACC and PA cases were positive for EZH2 and the ACC samples showed significantly higher EZH2 expression. The clinical and microscopic covariates were described in relation to EZH2 staining in ACC samples. The highest mean values of EZH2 were observed in cases with local metastasis, recurrence, perineural invasion, and predominantly cribriform growth pattern without solid areas. EZH2 is a potential marker of malignancy.

Carcinoma de células escamosas de boca: relação entre graduação histopatológica e características clínicas da neoplasia / Oral squamous cell carcinoma: relation between histopathologic graduation and clinical features of the neoplasia

As características histopatológicas do carcinoma de células escamosas de boca (CCEB) têm sido utilizadas para previsão do comportamento clínico desta neoplasia. Objetivo: Descrever as características de uma amostra de CCEB e verificar a relação da graduação histopatológica com características do indivíduo e da lesão, procurando identificar associações relacionadas com o comportamento agressivo desta neoplasia. Métodos: O protocolo foi aprovado pelo Comitê de Ética em Pesquisa da UFMG. Todos os casos selecionados foram de CCEB diagnosticados no serviço de Patologia Bucal da Faculdade de Odontologia da UFMG e no serviço de Estomatologia do Hospital Municipal Odilon Behrens. A amostra compreendeu 72 casos de CCEB, sendo 56 homens e 16 mulheres. Os indivíduos foram submetidos a questionários quanto aos seus hábitos tabagista e etilista, tempo de evolução da lesão e sintomatologia. Foram realizados exames clínicos, e os fragmentos tumorais foram revisados e graduados através do método proposto por Bryne et al. (1989). Os dados foram analisados, pelo teste do Qui-quadrado, no software SPSS 12.0 (p menor que 0,05). Resultados: Os tumores foram graduados como 23 bem diferenciados (32%), 23 moderadamente diferenciados (32%) e 26 mal diferenciados (36%). Neoplasias histologicamente mais agressivas foram mais frequentemente localizadas em língua ou assoalho bucal, sem, entretanto diferença estatisticamente significante (p maior que 0,05). A maioria das lesões mal diferenciadas (15 / 71,4%) exibiu metástase regional e o envolvimento linfonodal mostrou associação com tamanho da lesão (p menor que 0,05). Conclusão: Os resultados sugerem que algumas características da neoplasia, tais como, tamanho do tumor, sua localização (língua e/ou assoalho bucal), bem como a ocorrência de metástases para linfonodos locais, podem estar relacionados com a agressividade histológica do CCEB.

The histopathological characteristics of oral squamous cell carcinoma (OSCC) have been used to predict the clinical behavior of this neoplasia. Purpose: The aim of this study was describe the characteristics of an OSCC sample and verify the relation between histological grading and characteristics of the subjects and the tumor, identifying associations with the aggressive behavior of this lesion. Method: The protocol of this study was approved by the Research Ethics Committee from Universidade Federal de Minas Gerais (COEP-UFMG). OSCC cases selected were diagnosed in Oral Diagnosis Service at the Dentistry School of UFMG and in Odilon Behrens Hospital. ). The sample consisted of 72 patients with OSCC, been 56 men and 16 women. The patients were submitted to a questionnaire about tobacco and alcohol habits, evolution time and symptomatology. Clinical examinations were performed and tumor fragments were reviewed and graded in accordance to the criteria proposed by Bryne et al. (1989). The data were analyzed in SPSS (Statistic Package for Social Sciences) 12.0 for Windows (p less than 0,05). Results: Twenty-three tumors were graded histologically as well differentiated (32%), 23 moderately differentiated (32%) and 26 poorly differentiated (36%). Neoplasms histologically more aggressive were often located in tongue and floor of mouth, however, without statistical significance (p greater than 0,05). The majority of the well differentiated lesions (71,4%) exhibited regional metastasis and the lymph node involvement showed association with tumor's size (p less than 0,05). Conclusion: The results suggest that histological aggressiveness could be associated with size and regional dissemination in OSCC and that tumors located in tongue and floor of mouth may exhibit more aggressive behavior.

Lesão liquenóide oral relacionada ao amálgama / Amalgam-related oral lichenoid lesion

A lesão liquenóide oral relacionada ao amálgama, condição rara na prática odontológica, constitui importante diagnóstico diferencial no grupo das leucoplasias orais. Relatam-se dois casos que apresentaram rápida resolução clínica após a substituição das restaurações de amálgama.

Amalgam-related oral lichenoid lesion, a rare disorder in dental practice, is an important differential diagnosis among oral leukoplastic lesions. We report two cases with rapid clinical resolution following the replacement of amalgam fillings.